Selected Publications

• Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches. Kim MK, Suh GA, Cullen GD, Perez Rodriguez S, Dharmaraj T, Chang THW, Li Z, Chen Q, Green SI, Lavigne R, Pirnay JP, Bollyky PL, Sacher JC. JOURNAL OF CLINICAL INVESTIGATION. 2025 Mar 3;135(5):e187996. doi: 10.1172/JCI187996. PMID: 40026251
• Identification of bacteriophage DNA in human umbilical cord blood. JA Sequioa, NL Haddock, PM Gay, LJ Barkal, P Narasimhan, N Martinez, VD Winn, and PL Bollyky. JCI INSIGHT. 2025;10(13):e183123. https://doi.org/10.1172/jci.insight.183123. 
• Prophages are infrequently associated with antibiotic resistance in Pseudomonas aeruginosa clinical isolates. Chang TH, Pourtois JD, Haddock NL, Furukawa D, Kelly KE, Amanatullah DF, Burgener E, Milla C, Banaei N, Bollyky PL. mSPHERE. 2025 Mar 25;10(3):e0090424. doi: 10.1128/msphere.00904-24. PMID: 39945525 
• A blueprint for broadly effective bacteriophage-antibiotic cocktails against bacterial infections. MK Kim, Q Chen, A Echterhof, N Pennetzdorfer, RC McBride, N Banaei, Banaei N, Burgener EB, Milla CE, Bollyky PL. NATURE COMMUNICATIONS, 2024. Nov 28;15(1):9987. doi: 10.1038/s41467-024-53994-9.PMID: 39609398 
• Pf bacteriophages hinder sputum antibiotic diffusion via electrostatic binding. Chen Q, Cai P, Chang THW, Burgener E, Kratochvil MJ, Gupta A, Hargill A, Secor PR, Nielsen JE, Barron AE, Milla C, Heilshorn SC, Spakowitz A, Bollyky PL. SCIENCE ADVANCES. 2024 May 31;10(22):eadl5576. doi: 10.1126/sciadv.adl5576. PMID: 38820163 
• Regulatory T cells use heparanase to access IL-2 bound to extracellular matrix in inflamed tissue. Martinez HA, Koliesnik I, Kaber G, Reid JK, Nagy N, Barlow G, Falk BA, Medina CO, Hargil A, Zihsler S, Vlodavsky I, Li JP, Pérez-Cruz M, Tang SW, Meyer EH, Wrenshall LE, Lord JD, Garcia KC, Palmer TD, Steinman L, Nepom GT, Wight TN, Bollyky PL*, Kuipers HF*. NATURE COMMUNICATIONS. 2024 Feb 20;15(1):1564. doi: 10.1038/s41467-024-45012-9. *Co-senior authors
• The contribution of neutrophils to bacteriophage clearance and pharmacokinetics in vivo. Echterhof A, Dharmaraj T, Khosravi A, McBride R, Miesel L, Chia JH, Blankenberg PM, Lin KY, Shen CC, Lee YL, Yeh YC, Liao WT, Blankenberg FG, Dąbrowska K, Amanatullah DF, Frymoyer AR, Bollyky PL. JCI INSIGHT. 2024 Oct 22;9(20):e181309. doi: 10.1172/jci.insight.181309.PMID: 39435664
• The lysogenic filamentous Pseudomonas bacteriophage phage Pf slows mucociliary transport. Burgener EB, Cai PC, Kratochvil MJ, Rojas-Hernandez LS, Joo NS, Gupta A, Secor PR, Heilshorn SC, Spakowitz AJ, Wine JJ, Bollyky PL, Milla CE. PNAS Nexus. 2024 Sep 9;3(9):pgae390. doi: 10.1093/pnasnexus/pgae390. eCollection 2024 Sep.PMID: 39301510 
• Targeted deletion of Pf prophages from diverse Pseudomonas aeruginosa isolates has differential impacts on quorum sensing and virulence traits. Schmidt AK, Schwartzkopf CM, Pourtois JD, Burgener EB, Faith DR, Joyce A, Lamma T, Kumar G, Bollyky PL, Secor PR. J BACTERIOLOGY. 2024 May 23;206(5):e0040223. doi: 10.1128/jb.00402-23. Epub 2024 Apr 30.PMID: 38687034 
• The Inovirus Pf4 Triggers Antiviral Responses and Disrupts the Proliferation of Airway Basal Epithelial Cells. Popescu MC, Haddock NL, Burgener EB, Rojas-Hernandez LS, Kaber G, Hargil A, Bollyky PL, Milla CE. VIRUSES. 2024 Jan 22;16(1):165. doi: 10.3390/v16010165.PMID: 38275975 
• Bacterial outer membrane vesicles bound to bacteriophages modulate neutrophil responses to bacterial infection. Pennetzdorfer N, Popescu MC, Haddock NL, Dupuy F, Kaber G, Hargil A, Johansson PK, Enejder A, Bollyky PL. FRONTIERS CELL AND INFECTION MICROBIOLOGY. 2023 Oct 26;13:1250339. doi: 10.3389/fcimb.2023.1250339. eCollection 2023. PMID: 37965262
• Rapid assessment of changes in phage bioactivity using dynamic light scattering T Dharmaraj, MJ Kratochvil, JD Pourtois, Q Chen, M Hajfathalian, A Hargil, YH Lin, Z Evans, A Oromí-Bosch, JD Berry, R McBride, NL Haddock, DR Holman, JD Van Belleghem, TH Chang, JJ Barr, R Lavigne, SC Heilshorn, FG Blankenberg, and PL Bollyky. PNAS NEXUS 2 (12), 2023 pgad406
• Phage diversity in cell-free DNA identifies bacterial pathogens in human sepsis cases. NL Haddock, LJ Barkal, N Ram-Mohan, G Kaber, CY Chiu, AS Bhatt, S Yang, PL Bollyky. NATURE MICROBIOLOGY. 2023; doi.org/10.1038/s41564-023-01406-x
• Bacteriophage populations mirror those of bacterial pathogens at sites of infection. Haddock NL, Barkal LJ, Bollyky PL.mSystems. 2023 Aug 1:e0049723. mSYSTEMS. doi: 10.1128/msystems.00497-23.
• Bacteriophage DNA in blood provides species-level insight into bacterial infections NATURE MICROBIOLOGY Haddock, N. L., Bollyky, P. L.2023 doi.org/10.1038/s41564-023-01422-x
• Filamentous bacteriophage delays healing of Pseudomonas-infected wounds. Bach MS, de Vries CR, Khosravi A, Sweere JM, Popescu MC, Chen Q, Demirdjian S, Hargil A, Van Belleghem JD, Kaber G, Hajfathalian M, Burgener EB, Liu D, Tran QL, Dharmaraj T, Birukova M, Sunkari V, Balaji S, Ghosh N, Mathew-Steiner SS, El Masry MS, Keswani SG, Banaei N, Nedelec L, Sen CK, Chandra V, Secor PR, Suh GA, Bollyky PL. CELL REPORTS. 2022 Jun 21;3(6):100656. doi: 10.1016/j.xcrm.2022.100656. PMID: 35732145
• Bacteriophages and the Immune System. Popescu M, Van Belleghem JD, Khosravi A, Bollyky PL. ANNU REV VIROL. 2021 May. PMID: 34014761
• Plasma Microbial Cell-free DNA Next-generation Sequencing in the Diagnosis and Management of Febrile Neutropenia. E Benamu, K Gajurel, JN Anderson, T Lieb, CA Gomez, H Seng, R Aquino, D Hollemon, D Hong, T Blauwkamp, M Kertesz, L Blair, PL Bollyky, BC Medeiros, S Coutre, S Zompi, JG Montoya, S Deresinski. CLINICAL INFECTIOUS DISEASES 2021 Apr 19:ciab324. doi: 10.1093/cid/ciab324. PMID: 33870413.
• Phage Therapy for Limb-threatening Prosthetic Knee Klebsiella pneumoniae Infection: Case Report and In Vitro Characterization of Anti-biofilm Activity. Cano EJ, Caflisch KM, Bollyky PL, Van Belleghem JD, Patel R, Fackler J, Brownstein MJ, Horne B, Biswas, Henry M, Malagon F, Lewallen DG, Suh GA. CLINICAL INFECTIOUS DISEASES. 2020 Jul. PMID: 32699879
• The immune response to chronic Pseudomonas aeruginosa wound infection in immune-competent mice. JM Sweere, H Ishak, V Sunkari, MS Bach, R Manasherob,K Yadava, SM Ruppert, CK Sen, S Balaji, SG Keswani, PR Secor, and PL Bollyky. ADVANCES IN WOUND CARE. 2020 Feb 1;9(2):35-47. doi: 10.1089/wound.2019.1039. PMID:31903297
• Filamentous Bacteriophage Suppress Clearance of Bacterial Infection. JM Sweere, J Van Bellegehem, H Ishak, MS Bach, M Popescu, V Sunkari, G Kaber, R Manasherob,GA Suh, X Cao,CR De Vries, DN Lam, PL Marshall, M Birukova, E Katznelson, DV Lazzareschi, S Balaji, S Keswani, TR Hawn, PR Secor, and PL Bollyky.^. SCIENCE. (2019) 363, 6434, 1416. DOI: 10.1126/science.aat9691
• Filamentous bacteriophages are associated with chronic Pseudomonas lung infections and antibiotic resistance in Cystic Fibrosis. EB Burgener, JM Sweere, MS Bach, PR Secor, N Haddock, LK Jennings, RL Marvig, HK Johansen, E Rossi, X Cao, L Tian, L Nedelec, S Molin, PL Bollyky* and CE Milla*  SCIENCE TRANSLATIONAL MEDICINE. 17 Apr 2019: Vol. 11, Issue 488, eaau9748 DOI: 10.1126/scitranslmed.aau9748   *co-senior authors
• The innate sense of bacteriophages. Bollyky PL, Secor PR. CELL HOST & MICROBE. 2019 Feb 13;25(2):177-179. doi: 10.1016/j.chom.2019.01.020.
• Macrophages and innate immune memory against Staphylococcus skin infections. Van Belleghem JD and Bollyky PL. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES. Nov 2018, 201816935; DOI:10.1073/pnas.1816935115
• Interactions between Bacteriophage, Bacteria, and the Mammalian Immune System. Van Belleghem JD, Dąbrowska K, Vaneechoutte M, Barr JJ, Bollyky PL. VIRUSES. 2018 Dec 25;11(1). pii: E10. doi: 10.3390/v11010010.
• The Immune Response to Chronic Pseudomonas aeruginosa Wound Infection in Immunocompetent Mice. JM Sweere, H Ishak, V Sunkari, MS Bach, R Manasherob, K Yadava, SM Ruppert, CK Sen, S Balaji, SG Keswani, PR Secor, and PL Bollyky.  ADVANCES IN WOUND CARE.  2019 6 Aug 2019. doi.org/10.1089/wound.2019.1039
• Pf4 bacteriophage produced by Pseudomonas aeruginosa inhibits Aspergillus fumigatus metabolism via iron sequestration. Penner, JC., Ferreira, JA, Secor, PR, Sweere, JM, Birukova, M, Joubert, LM, Haagensen, JA, Garcia, O., Malkovskiy, AV., Kaber, G, Nazik, H., Manasherob, R, Spormann, AM, Clemons, KV., Stevens, D.A, Bollyky, PL. 2016 MICROBIOLOGY.  DOI: 10.1099/mic.0.000344
• PR Secor, JM Sweere, LA Michaels, AV Malkovskiy, J Rajadas, D Lazzareschi, E Katznelson, A Arrigoni, KR Braun, SP Evanko, W Kaminsky, PK Singh, WC Parks, and PL Bollyky^. Filamentous bacteriophage promote biofilm assembly and antibiotic tolerance. CELL HOST & MICROBE. 2015, Nov 11;18(5):549-59. PMID: 26567508